Hotspot

trnG-UCC-trnM-CAU

LSC · 13692-15694 · 2003 bp

Back to Iseilema

Pi 0.00375
MarkerSeek score 52.90
Primer available yes
Label trnG-UCC-trnM-CAU

Feature Metadata

label_name
trnG-UCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
2003
variable_sites
17
pi
0.00375
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnM-CAU_p1 ACGAATCACACTTTTACCACT TCGAACCGATGACTTCTGTC 38.1 50.0 55.5 57.4 1981-2222 bp 1.0 51.3
trnG-UCC-trnM-CAU_p2 ACGAATCACACTTTTACCACT CGAACCGATGACTTCTGTCT 38.1 50.0 55.5 57.4 1980-2221 bp 1.0 51.2
trnG-UCC-trnM-CAU_p3 AGAACGAATCACACTTTTACCAC TCGAACCGATGACTTCTGTC 39.1 50.0 57.3 57.4 1984-2225 bp 1.0 50.2
trnG-UCC-trnM-CAU_p4 GAACGAATCACACTTTTACCACT TCGAACCGATGACTTCTGTC 39.1 50.0 57.3 57.4 1983-2224 bp 1.0 50.2
trnG-UCC-trnM-CAU_p5 GAACGAATCACACTTTTACCACT CGAACCGATGACTTCTGTCT 39.1 50.0 57.3 57.4 1982-2223 bp 1.0 50.2