Hotspot

psaJ-rpl33

LSC · 66261-66701 · 441 bp

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Pi 0.0291
MarkerSeek score 70.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
441
variable_sites
36
pi
0.0291
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTCGGTTCGTTAGCAGGTTT ATATCTGGAAATTCCCGCCG 45.0 50.0 57.8 57.8 601-618 bp 1.0 84.4
psaJ-rpl33_p2 ATGGTTCGGTTCGTTAGCAG ATATCTGGAAATTCCCGCCG 50.0 50.0 58.3 57.8 605-622 bp 1.0 84.1
psaJ-rpl33_p3 TTCGGTTCGTTAGCAGGTTT CCCGCCGATTCCTTCTTAAT 45.0 50.0 57.8 57.7 588-605 bp 1.0 84.0
psaJ-rpl33_p4 ATGGTTCGGTTCGTTAGCAG CCCGCCGATTCCTTCTTAAT 50.0 50.0 58.3 57.7 592-609 bp 1.0 83.7
psaJ-rpl33_p5 GTTCGGTTCGTTAGCAGGTT ATATCTGGAAATTCCCGCCG 50.0 50.0 58.5 57.8 602-619 bp 1.0 82.4