Hotspot

trnT-CGU-trnR-UCU

LSC · 9543-9703 · 161 bp

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Pi 0.0393
MarkerSeek score 65.40
Primer available yes
Label trnT-CGU-trnR-UCU

Feature Metadata

label_name
trnT-CGU-trnR-UCU
feature_type
igs
region
LSC
length_bp
161
variable_sites
15
pi
0.0393
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-CGU-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 245-251 bp 1.0 50.6
trnT-CGU-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 239-245 bp 1.0 50.5
trnT-CGU-trnR-UCU_p3 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTATGTCCT 50.0 40.9 58.0 55.6 251-257 bp 1.0 50.4
trnT-CGU-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGAAGACCTATGTCCTATCCA 50.0 42.9 58.0 54.6 245-251 bp 1.0 50.3
trnT-CGU-trnR-UCU_p5 CGACGTCGACTAAAACCCTT AGGTTTAGAAGACCTATGTCCT 50.0 40.9 57.9 55.6 265-271 bp 1.0 50.0