Hotspot

rps16-trnQ-UUG

LSC · 6462-6913 · 452 bp

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Pi 0.0191
MarkerSeek score 58.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
452
variable_sites
26
pi
0.0191
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA CTTACAGCTGCTTGCCAAAC 45.0 50.0 58.1 57.9 1067-1099 bp 1.0 83.7
rps16-trnQ-UUG_p2 AAACGCTTGATTCACGCATG GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 867-899 bp 0.888889 81.3
rps16-trnQ-UUG_p3 CAAACGCTTGATTCACGCAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 868-900 bp 0.888889 81.3
rps16-trnQ-UUG_p4 AAACGCTTGATTCACGCATG CTTACAGCTGCTTGCCAAAC 45.0 50.0 58.0 57.9 1431-1466 bp 0.888889 79.8
rps16-trnQ-UUG_p5 CAAACGCTTGATTCACGCAT CTTACAGCTGCTTGCCAAAC 45.0 50.0 58.0 57.9 1432-1467 bp 0.888889 79.8