primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	57.9	58.1	0.0	12.9	0.188044	77	644	341-673	1.000000	90.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	77	644	356-688	1.000000	90.4
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	644	359-691	1.000000	90.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	20	20	50.0	55.0	58.2	58.1	0.0	12.9	0.213831	77	644	342-674	1.000000	90.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	58.2	57.9	0.0	13.4	0.226475	77	644	357-689	1.000000	90.2
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	CTTACAGCTGCTTGCCAAAC	20	20	45.0	50.0	58.1	57.9	8.1	15.3	0.195418	6462	6913	1067-1099	1.000000	83.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAACGCTTGATTCACGCATG	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	14.6	16.9	0.108339	6462	6913	867-899	0.888889	81.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	CAAACGCTTGATTCACGCAT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.0	58.1	14.6	16.9	0.108339	6462	6913	868-900	0.888889	81.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAACGCTTGATTCACGCATG	CTTACAGCTGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	14.6	15.3	0.088333	6462	6913	1431-1466	0.888889	79.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CAAACGCTTGATTCACGCAT	CTTACAGCTGCTTGCCAAAC	20	20	45.0	50.0	58.0	57.9	14.6	15.3	0.088333	6462	6913	1432-1467	0.888889	79.8
psbI-trnS-GCU_p1	psbI-trnS-GCU	1	TCCTATCTAATGATCCAGGACGT	TAGGAGAGATGGCTGAGTGG	23	20	43.5	55.0	58.0	57.9	0.0	0.0	3.079860	7913	7997	209-224	1.000000	61.6
psbI-trnS-GCU_p2	psbI-trnS-GCU	2	TCCTATCTAATGATCCAGGACGT	TGAGTGGACTAAAGCGTTGG	23	20	43.5	50.0	58.0	57.8	0.0	0.0	3.164508	7913	7997	196-211	1.000000	61.3
psbI-trnS-GCU_p3	psbI-trnS-GCU	3	TCCTATCTAATGATCCAGGACGT	GCTGAGTGGACTAAAGCGTT	23	20	43.5	50.0	58.0	58.2	0.0	0.0	3.203666	7913	7997	198-213	1.000000	61.0
psbI-trnS-GCU_p4	psbI-trnS-GCU	4	TCCTATCTAATGATCCAGGACGT	CGAGTTAATCGTACCGAGGG	23	20	43.5	55.0	58.0	57.6	0.0	0.0	3.396850	7913	7997	159-174	1.000000	60.2
psbI-trnS-GCU_p5	psbI-trnS-GCU	5	TCCTATCTAATGATCCAGGACGT	ATGGCTGAGTGGACTAAAGC	23	20	43.5	50.0	58.0	57.6	0.0	5.7	3.410639	7913	7997	201-216	1.000000	59.2
trnS-GCU-trnT-CGU_p1	trnS-GCU-trnT-CGU	1	CCAACGCTTTAGTCCACTCA	AGGGGTTAAAGGAACGAATCA	20	21	50.0	42.9	57.8	56.9	0.0	0.0	2.245977	8086	8753	691-840	1.000000	72.0
trnS-GCU-trnT-CGU_p2	trnS-GCU-trnT-CGU	2	CCAACGCTTTAGTCCACTCA	GGGGTTAAAGGAACGAATCAC	20	21	50.0	47.6	57.8	56.9	0.0	0.0	2.280588	8086	8753	690-839	1.000000	71.8
trnS-GCU-trnT-CGU_p3	trnS-GCU-trnT-CGU	3	AACGCTTTAGTCCACTCAGC	AGGGGTTAAAGGAACGAATCA	20	21	50.0	42.9	58.2	56.9	2.0	0.0	2.285135	8086	8753	689-838	1.000000	71.7
trnS-GCU-trnT-CGU_p4	trnS-GCU-trnT-CGU	4	AACGCTTTAGTCCACTCAGC	GGGGTTAAAGGAACGAATCAC	20	21	50.0	47.6	58.2	56.9	2.0	0.0	2.319746	8086	8753	688-837	1.000000	71.5
trnS-GCU-trnT-CGU_p5	trnS-GCU-trnT-CGU	5	CCAACGCTTTAGTCCACTCA	AGGGGTTAAAGGAACGAATCAC	20	22	50.0	45.5	57.8	58.3	0.0	0.0	2.481122	8086	8753	691-840	1.000000	70.1
trnT-CGU-trnR-UCU_p1	trnT-CGU-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.404255	9543	9703	245-251	1.000000	50.6
trnT-CGU-trnR-UCU_p2	trnT-CGU-trnR-UCU	2	AGCCTTCCAAGCTAACGATG	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.0	54.6	0.0	0.0	4.434337	9543	9703	239-245	1.000000	50.5
trnT-CGU-trnR-UCU_p3	trnT-CGU-trnR-UCU	3	ACCCTTAGCCTTCCAAGCTA	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	58.0	55.6	0.0	0.0	4.407572	9543	9703	251-257	1.000000	50.4
trnT-CGU-trnR-UCU_p4	trnT-CGU-trnR-UCU	4	ACCCTTAGCCTTCCAAGCTA	AGAAGACCTATGTCCTATCCA	20	21	50.0	42.9	58.0	54.6	0.0	0.0	4.437654	9543	9703	245-251	1.000000	50.3
trnT-CGU-trnR-UCU_p5	trnT-CGU-trnR-UCU	5	CGACGTCGACTAAAACCCTT	AGGTTTAGAAGACCTATGTCCT	20	22	50.0	40.9	57.9	55.6	33.4	0.0	4.430511	9543	9703	265-271	1.000000	50.0
trnE-trnT-GGU_p1	trnE-trnT-GGU	1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.269359	30649	31470	899-929	1.000000	86.3
trnE-trnT-GGU_p2	trnE-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	30649	31470	878-908	1.000000	86.2
trnE-trnT-GGU_p3	trnE-trnT-GGU	3	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.3	58.1	0.0	0.0	0.327456	30649	31470	878-908	1.000000	85.9
trnE-trnT-GGU_p4	trnE-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.345606	30649	31470	857-887	1.000000	85.9
trnE-trnT-GGU_p5	trnE-trnT-GGU	5	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.349611	30649	31470	875-905	1.000000	85.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.283643	46184	46944	675-823	1.000000	85.7
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	46184	46944	678-826	1.000000	84.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.391397	46184	46944	707-855	1.000000	84.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	20	20	50.0	50.0	57.8	58.2	15.5	10.2	0.480302	46184	46944	710-858	1.000000	83.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCA	20	20	55.0	50.0	57.9	57.6	0.0	10.2	0.534323	46184	46944	680-828	1.000000	83.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TTTCTCTGGCCCCTTCCTAA	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.129726	50037	50719	825-1026	1.000000	85.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTTCTCTGGCCCCTTCCTAA	CAGGTCTACGGTTCGAATCC	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.259054	50037	50719	834-1035	1.000000	84.5
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TTTCTCTGGCCCCTTCCTAA	CGGTTCGAATCCGTATAGCC	20	20	50.0	55.0	57.9	58.3	0.0	22.8	0.359107	50037	50719	826-1027	1.000000	83.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTCTGGCCCCTTCCTAATT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.362914	50037	50719	823-1024	1.000000	83.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TTCTCTGGCCCCTTCCTAAT	GGTTCGAATCCGTATAGCCC	20	20	50.0	55.0	57.7	57.9	0.0	0.0	0.362914	50037	50719	824-1025	1.000000	83.8
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	GGCAGGAGTCATTGGTTCAA	AGTCGAGGCTCTCAATACGA	20	20	50.0	50.0	58.1	58.0	0.0	13.5	0.130118	51664	51800	142-206	1.000000	88.1
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	GGCAGGAGTCATTGGTTCAA	ACGAGTCGAGGCTCTCAATA	20	20	50.0	50.0	58.1	58.0	0.0	21.5	0.130118	51664	51800	145-209	1.000000	88.0
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	TTGCTTTCATACGGCAGGAG	AGTCGAGGCTCTCAATACGA	20	20	50.0	50.0	58.3	58.0	0.0	13.5	0.301980	51664	51800	154-218	1.000000	86.4
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	TTGCTTTCATACGGCAGGAG	ACGAGTCGAGGCTCTCAATA	20	20	50.0	50.0	58.3	58.0	0.0	21.5	0.301980	51664	51800	157-221	1.000000	86.3
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	CATACGGCAGGAGTCATTGG	AGTCGAGGCTCTCAATACGA	20	20	55.0	50.0	58.4	58.0	0.0	13.5	0.453396	51664	51800	147-211	1.000000	85.4
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	GAAGCGGGATAAATGGCTGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.071068	61855	62810	1186-1231	1.000000	87.1
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	AACAGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.0	7.1	0.043011	61855	62810	1639-1684	1.000000	86.4
petA-psbJ_p3	petA-psbJ	3	ATCCATTACGTGTCCAAGGC	ACAGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.043011	61855	62810	1638-1683	1.000000	86.4
petA-psbJ_p4	petA-psbJ	4	GTTTCAGAGGGCGAATCCAT	AACAGGCCGTTTTGATCCTT	20	20	50.0	45.0	58.0	58.0	0.0	7.1	0.048746	61855	62810	1736-1781	1.000000	86.1
petA-psbJ_p5	petA-psbJ	5	GTTTCAGAGGGCGAATCCAT	ACAGGCCGTTTTGATCCTTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.048746	61855	62810	1735-1780	1.000000	86.1
psbE-petL_p1	psbE-petL	1	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	57.9	56.3	0.0	12.5	1.783771	63596	64959	1231-1682	1.000000	73.3
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	AGCTGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.3	24.9	12.5	1.868337	63596	64959	1149-1600	1.000000	72.9
psbE-petL_p3	psbE-petL	3	AATAACCAGCCCGCAATGAA	AGCTGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.3	0.0	12.5	1.865987	63596	64959	1232-1683	1.000000	72.7
psbE-petL_p4	psbE-petL	4	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	20	20	55.0	45.0	58.2	56.3	0.9	12.5	1.909925	63596	64959	1144-1595	1.000000	72.5
psbE-petL_p5	psbE-petL	5	ATAACCAGCCCGCAATGAAT	AGCTGCTAGTAGAAAACCGAA	20	21	45.0	42.9	57.9	57.0	0.0	12.5	2.027428	63596	64959	1231-1682	1.000000	71.4
psaJ-rpl33_p1	psaJ-rpl33	1	TTCGGTTCGTTAGCAGGTTT	ATATCTGGAAATTCCCGCCG	20	20	45.0	50.0	57.8	57.8	0.0	9.9	0.428606	66261	66701	601-618	1.000000	84.4
psaJ-rpl33_p2	psaJ-rpl33	2	ATGGTTCGGTTCGTTAGCAG	ATATCTGGAAATTCCCGCCG	20	20	50.0	50.0	58.3	57.8	0.0	9.9	0.462993	66261	66701	605-622	1.000000	84.1
psaJ-rpl33_p3	psaJ-rpl33	3	TTCGGTTCGTTAGCAGGTTT	CCCGCCGATTCCTTCTTAAT	20	20	45.0	50.0	57.8	57.7	0.0	0.0	0.499719	66261	66701	588-605	1.000000	84.0
psaJ-rpl33_p4	psaJ-rpl33	4	ATGGTTCGGTTCGTTAGCAG	CCCGCCGATTCCTTCTTAAT	20	20	50.0	50.0	58.3	57.7	0.0	0.0	0.534106	66261	66701	592-609	1.000000	83.7
psaJ-rpl33_p5	psaJ-rpl33	5	GTTCGGTTCGTTAGCAGGTT	ATATCTGGAAATTCCCGCCG	20	20	50.0	50.0	58.5	57.8	0.0	9.9	0.679579	66261	66701	602-619	1.000000	82.4
rpl36-rps8_p1	rpl36-rps8	1	TTTTACGAACGGAAGCCCTT	GGCGGAGAAATTTTGTGTTA	20	20	45.0	40.0	57.7	54.5	0.0	0.0	3.761136	78930	79398	523-541	1.000000	57.0
rpl36-rps8_p2	rpl36-rps8	2	ACGAACGGAAGCCCTTATTT	GGCGGAGAAATTTTGTGTTA	20	20	45.0	40.0	57.5	54.5	0.0	0.0	3.986611	78930	79398	519-537	1.000000	55.3
rpl36-rps8_p3	rpl36-rps8	3	ATTTTACGAACGGAAGCCCT	GGCGGAGAAATTTTGTGTTA	20	20	45.0	40.0	57.5	54.5	0.0	0.0	3.986611	78930	79398	524-542	1.000000	55.2
rpl36-rps8_p4	rpl36-rps8	4	TTTTACGAACGGAAGCCCTT	GGCGGAGAAATTTTGTGTTAT	20	21	45.0	38.1	57.7	55.1	0.0	0.0	4.153809	78930	79398	523-541	1.000000	53.9
rpl36-rps8_p5	rpl36-rps8	5	ACGAACGGAAGCCCTTATTT	GGCGGAGAAATTTTGTGTTAT	20	21	45.0	38.1	57.5	55.1	0.0	0.0	4.379285	78930	79398	519-537	1.000000	52.1
ndhF-rpl32_p1	ndhF-rpl32	1	TCCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGA	27	20	29.6	40.0	56.7	55.5	0.0	19.5	10.706462	112023	112831	878-895	1.000000	47.7
ndhF-rpl32_p2	ndhF-rpl32	2	CCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGA	26	20	30.8	40.0	55.5	55.5	0.0	19.5	10.945020	112023	112831	877-894	1.000000	47.7
ndhF-rpl32_p3	ndhF-rpl32	3	TCCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGAT	27	21	29.6	38.1	56.7	56.1	0.0	19.5	11.150403	112023	112831	878-895	1.000000	47.7
ndhF-rpl32_p4	ndhF-rpl32	4	TCCATGAATATTGATATGTATGTTCC	ACGAATACGCTTTTTCGAGA	26	20	30.8	40.0	55.3	55.5	0.0	19.5	11.174465	112023	112831	878-895	1.000000	47.7
ndhF-rpl32_p5	ndhF-rpl32	5	CCATGAATATTGATATGTATGTTCCA	ACGAATACGCTTTTTCGAGAT	26	21	30.8	38.1	55.5	56.1	0.0	19.5	11.388961	112023	112831	877-894	1.000000	47.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TGGACATCGTTGAAAGCCTT	TGCAGACTGAGGCATTTTGT	20	20	45.0	45.0	57.7	58.0	0.0	6.7	0.284879	112991	113704	1347-1383	1.000000	88.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TTGGACATCGTTGAAAGCCT	TGCAGACTGAGGCATTTTGT	20	20	45.0	45.0	57.7	58.0	0.0	6.7	0.284879	112991	113704	1348-1384	1.000000	88.8
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TGGACATCGTTGAAAGCCTT	TAACCACTGGGACTGGAGAG	20	20	45.0	55.0	57.7	58.1	0.0	0.0	0.331767	112991	113704	1380-1416	1.000000	88.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TGGACATCGTTGAAAGCCTT	TGATCCACATAACAGAGCGC	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.330378	112991	113704	1434-1470	1.000000	88.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TTGGACATCGTTGAAAGCCT	TGATCCACATAACAGAGCGC	20	20	45.0	50.0	57.7	58.1	0.0	0.0	0.330378	112991	113704	1435-1471	1.000000	88.0
ycf1_p1	ycf1	1	AGCCCCCAAACCGTTAAAAA	CGTTCCCAAAGGCGTAAAAC	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.249896	126220	126339	608-608	1.000000	85.4
ycf1_p2	ycf1	2	AGCCCCCAAACCGTTAAAAA	GGATCTCTCGCGGAAGAATC	20	20	45.0	55.0	58.2	57.9	0.0	6.1	0.268095	126220	126339	1192-1198	1.000000	85.0
ycf1_p3	ycf1	3	AGTCGAGCCTTCTGAGATGA	CGTTCCCAAAGGCGTAAAAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.177013	126220	126339	554-554	0.888889	80.8
ycf1_p4	ycf1	4	AGTCGAGCCTTCTGAGATGA	GGATCTCTCGCGGAAGAATC	20	20	50.0	55.0	57.9	57.9	0.0	6.1	0.195212	126220	126339	1138-1144	0.888889	80.2
ycf1_p5	ycf1	5	AGTCGAGCCTTCTGAGATGA	GCCAGGACTTCGTTCAAGAA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.257345	126220	126339	732-732	0.666667	67.8