Hotspot

petD

LSC · 73469-74688 · 1220 bp

Back to Isachne

Pi 0.0115

MarkerSeek score 50.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1220
variable_sites: 14
pi: 0.0115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CAGTTCATCGATGGTCAGCA	TGCTGTATCCATGCCTGTTC	50.0	50.0	58.0	58.0	2510-2532 bp	1.0	80.9
petD_p2	CAGTTCATCGATGGTCAGCA	TTCCTTTTCTACACGCGGAG	50.0	50.0	58.0	57.9	2349-2371 bp	1.0	80.7
petD_p3	AAGCAAGGTATTTCGGGTCC	TGCTGTATCCATGCCTGTTC	50.0	50.0	57.9	58.0	2139-2161 bp	1.0	80.2
petD_p4	GCAAGGTATTTCGGGTCCTT	TGCTGTATCCATGCCTGTTC	50.0	50.0	57.9	58.0	2137-2159 bp	1.0	80.2
petD_p5	GCAAGGTATTTCGGGTCCTT	TTCCTTTTCTACACGCGGAG	50.0	50.0	57.9	57.9	1976-1998 bp	1.0	80.1