Hotspot

psaC-ndhE

SSC · 109087-109569 · 483 bp

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Pi 0.0232

MarkerSeek score 65.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 483
variable_sites: 11
pi: 0.0232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTTGGACAAGCTCGTACACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	658-666 bp	1.0	68.5
psaC-ndhE_p2	TGTTGGACAAGCTCGTACAC	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.1	56.4	659-667 bp	1.0	68.5
psaC-ndhE_p3	CTGTTGGACAAGCTCGTACA	TTTTGTTATAGCCCTTGCGG	50.0	45.0	57.9	56.4	660-668 bp	1.0	68.4
psaC-ndhE_p4	ACATCTGTTGGACAAGCTCG	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.2	56.4	664-672 bp	1.0	68.0
psaC-ndhE_p5	CATCTGTTGGACAAGCTCGT	TTTTGTTATAGCCCTTGCGG	50.0	45.0	58.2	56.4	663-671 bp	1.0	68.0