Hotspot

ndhF-rpl32

SSC · 104335-105198 · 864 bp

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Pi 0.0204

MarkerSeek score 65.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 864
variable_sites: 17
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGAAGTGGAAGAAGAGGGA	GCCACCGAAGTGCGTTTTT	50.0	52.6	57.3	59.9	921-947 bp	1.0	54.7
ndhF-rpl32_p2	AGGAAGTGGAAGAAGAGGGA	TTTTTGCCACCGAAGTGCG	50.0	52.6	57.3	59.9	926-952 bp	1.0	54.7
ndhF-rpl32_p3	AAGTCCAAACCCCATTGACA	GCCACCGAAGTGCGTTTTT	45.0	52.6	57.2	59.9	948-974 bp	1.0	54.2
ndhF-rpl32_p4	AAGTCCAAACCCCATTGACA	TTTTTGCCACCGAAGTGCG	45.0	52.6	57.2	59.9	953-979 bp	1.0	54.2
ndhF-rpl32_p5	CAGGAAGTGGAAGAAGAGGG	GCCACCGAAGTGCGTTTTT	55.0	52.6	57.2	59.9	922-948 bp	1.0	54.2