Hotspot

rpl14-rpl16

LSC · 78138-78225 · 88 bp

Back to Isachne

Pi 0.046

MarkerSeek score 72.60

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 88
variable_sites: 4
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AATTTTCGGGCTCCACTGTT	TAGCAGCCAGTAAAATGCCC	45.0	50.0	58.0	58.2	217-218 bp	1.0	79.9
rpl14-rpl16_p2	ACATCAATTTTCGGGCTCCA	TAGCAGCCAGTAAAATGCCC	45.0	50.0	57.8	58.2	222-223 bp	1.0	78.2
rpl14-rpl16_p3	AGCAGCTCCTATGACTCGAA	TAGCAGCCAGTAAAATGCCC	50.0	50.0	58.2	58.2	245-246 bp	1.0	77.9
rpl14-rpl16_p4	TTTCGGGCTCCACTGTTATC	TAGCAGCCAGTAAAATGCCC	50.0	50.0	57.6	58.2	214-215 bp	1.0	76.8
rpl14-rpl16_p5	TTCGGGCTCCACTGTTATCT	TAGCAGCCAGTAAAATGCCC	50.0	50.0	58.4	58.2	213-214 bp	1.0	76.5