Hotspot

clpP-psbB

LSC · 68955-69459 · 505 bp

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Pi 0.0119

MarkerSeek score 63.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 6
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTTTTGGAACACCAATCGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.9	57.9	629 bp	1.0	79.3
clpP-psbB_p2	ACCCAAGTCGCTTCTTCATC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.9	57.9	669 bp	1.0	79.0
clpP-psbB_p3	TCAACCCAAGTCGCTTCTTC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.1	57.9	672 bp	1.0	78.8
clpP-psbB_p4	CTTTTGGAACACCAATCGGC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	547 bp	1.0	78.5
clpP-psbB_p5	CTTTTGGAACACCAATCGGC	CAATCGACCCGGATCATTCA	50.0	50.0	57.9	57.8	584 bp	1.0	78.4