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atpI-atpH

LSC · 32167-32995 · 829 bp

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Pi 0.0134

MarkerSeek score 67.20

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 11
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	965 bp	1.0	75.8
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	898 bp	1.0	75.7
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	920 bp	1.0	75.6
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	905 bp	1.0	75.4
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	45.0	55.0	58.6	58.2	978 bp	1.0	75.3