Hotspot

trnC-GCA-rpoB

LSC · 18975-20131 · 1157 bp

Back to Isachne

Pi 0.0164

MarkerSeek score 61.20

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 19
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.1	58.4	1224-1251 bp	1.0	77.4
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TTCCCTCATTTCCATTCCGG	50.0	50.0	58.1	57.6	1225-1252 bp	1.0	77.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	TCCTTCGAATTGGACCTGAC	50.0	50.0	58.1	57.2	1266-1293 bp	1.0	74.5
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CCTTCGAATTGGACCTGACT	50.0	50.0	58.1	57.2	1265-1292 bp	1.0	74.5
trnC-GCA-rpoB_p5	TTTATTGATCAGGCGGCACC	TCCCTCATTTCCATTCCGGA	50.0	50.0	58.6	58.4	1262-1289 bp	1.0	73.0