Hotspot

psbM-petN

LSC · 17098-17870 · 773 bp

Back to Isachne

Pi 0.023

MarkerSeek score 69.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 17
pi: 0.023
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACGGCCAGCCAAAATGATTA	AGTATGGGGGAGAAGTGGAC	45.0	55.0	58.2	58.1	785-819 bp	1.0	83.0
psbM-petN_p2	ACGGCCAGCCAAAATGATTA	GTATGGGGGAGAAGTGGACT	45.0	55.0	58.2	58.1	784-818 bp	1.0	83.0
psbM-petN_p3	TATGTAAAAACGGCCAGCCA	AGTATGGGGGAGAAGTGGAC	45.0	55.0	57.8	58.1	794-828 bp	1.0	82.6
psbM-petN_p4	TATGTAAAAACGGCCAGCCA	GTATGGGGGAGAAGTGGACT	45.0	55.0	57.8	58.1	793-827 bp	1.0	82.6
psbM-petN_p5	ACGGCCAGCCAAAATGATTA	TATGGGGGAGAAGTGGACTC	45.0	55.0	58.2	57.8	783-817 bp	1.0	82.6