Hotspot

trnD-GUC-psbM

LSC · 16025-16992 · 968 bp

Back to Isachne

Pi 0.0166

MarkerSeek score 62.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 968
variable_sites: 16
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TAATCATTTTGGCTGGCCGT	50.0	45.0	58.5	58.2	1133-1136 bp	1.0	76.3
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	TGGCTGGCCGTTTTTACATA	50.0	45.0	58.5	57.8	1124-1127 bp	1.0	75.9
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	TTTTGGCTGGCCGTTTTTAC	50.0	45.0	58.5	57.8	1127-1130 bp	1.0	75.7
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	CATTTTGGCTGGCCGTTTTT	50.0	45.0	58.5	58.4	1129-1132 bp	1.0	74.3
trnD-GUC-psbM_p5	TTCAATTGGTCAGAGCACCG	TTGGCTGGCCGTTTTTACAT	50.0	45.0	58.5	58.7	1125-1128 bp	1.0	72.2