Hotspot

trnT-GGU-trnE-UUC

LSC · 15104-15564 · 461 bp

Back to Isachne

Pi 0.0174

MarkerSeek score 66.10

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 8
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	581-582 bp	1.0	68.3
trnT-GGU-trnE-UUC_p2	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	560-561 bp	1.0	68.2
trnT-GGU-trnE-UUC_p3	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	544-545 bp	1.0	68.2
trnT-GGU-trnE-UUC_p4	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	523-524 bp	1.0	68.1
trnT-GGU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	549-550 bp	1.0	67.5