Hotspot

trnG-UCC-trnT-GGU

LSC · 13319-15031 · 1713 bp

Back to Isachne

Pi 0.0158

MarkerSeek score 59.40

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1713
variable_sites: 27
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	ACGAATCACACTTTTACCACT	AGCACTAAACGAAAAGCCCT	38.1	45.0	55.5	57.7	1833-1844 bp	1.0	50.8
trnG-UCC-trnT-GGU_p2	ACGAATCACACTTTTACCACT	ACTTCCCTCTTACCATGGCA	38.1	50.0	55.5	58.3	1860-1871 bp	1.0	50.3
trnG-UCC-trnT-GGU_p3	AGAACGAATCACACTTTTACCAC	AGCACTAAACGAAAAGCCCT	39.1	45.0	57.3	57.7	1836-1847 bp	1.0	49.7
trnG-UCC-trnT-GGU_p4	GAACGAATCACACTTTTACCACT	AGCACTAAACGAAAAGCCCT	39.1	45.0	57.3	57.7	1835-1846 bp	1.0	49.7
trnG-UCC-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	ACTTCCCTCTTACCATGGCA	39.1	50.0	57.3	58.3	1862-1873 bp	1.0	49.2