Hotspot

trnS-GCU-psbD

LSC · 7643-8633 · 991 bp

Back to Isachne

Pi 0.0152

MarkerSeek score 57.20

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 991
variable_sites: 15
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	ACTCTACCAAGGGCTATAGTCA	45.0	45.5	58.0	57.5	1052-1054 bp	1.0	60.8
trnS-GCU-psbD_p2	GTCCCAAATGATCCAAGGGG	ACTCTACCAAGGGCTATAGTCA	55.0	45.5	58.2	57.5	1121-1123 bp	1.0	59.2
trnS-GCU-psbD_p3	GCTTTAGTCCACTCAGCCAT	ACTCTACCAAGGGCTATAGTCA	50.0	45.5	57.6	57.5	1038-1040 bp	1.0	57.8
trnS-GCU-psbD_p4	AGTCCACTCAGCCATCTCTC	ACTCTACCAAGGGCTATAGTCA	55.0	45.5	58.5	57.5	1033-1035 bp	1.0	57.0
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	ACTCTACCAAGGGCTATAGTCAT	45.0	43.5	58.0	57.9	1052-1054 bp	1.0	56.4