Hotspot

rps15-ycf1

SSC · 120879-121374 · 496 bp

Back to Iris

Pi 0.0648
MarkerSeek score 72.00
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
496
variable_sites
157
pi
0.0648
unique_haplotype_count
49
primer_available
yes
alignment_reliability
0.90
markerseek_score
72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 ATTCAACAGACCCGCAGTTT GACGTCGTAGAGAGTGGAGA 45.0 55.0 58.0 58.0 717-880 bp 0.903226 87.9
rps15-ycf1_p2 AATTCAACAGACCCGCAGTT GACGTCGTAGAGAGTGGAGA 45.0 55.0 58.0 58.0 718-881 bp 0.887097 87.3
rps15-ycf1_p3 ATTCAACAGACCCGCAGTTT AGACGTCGTAGAGAGTGGAG 45.0 55.0 58.0 58.0 718-881 bp 0.887097 87.2
rps15-ycf1_p4 AATTCAACAGACCCGCAGTT AGACGTCGTAGAGAGTGGAG 45.0 55.0 58.0 58.0 719-882 bp 0.870968 86.6
rps15-ycf1_p5 AAATTCAACAGACCCGCAGT GACGTCGTAGAGAGTGGAGA 45.0 55.0 58.0 58.0 821-882 bp 0.258065 58.6