Hotspot

rpl32-trnL-UAG

SSC · 110944-111688 · 745 bp

Back to Iris

Pi 0.0406
MarkerSeek score 74.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
745
variable_sites
199
pi
0.0406
unique_haplotype_count
54
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGGCAAACAAGT AGCGTGTCTACCGATTTCAC 40.0 50.0 57.9 58.0 740-1043 bp 0.887097 86.7
rpl32-trnL-UAG_p2 TTTTTGTGCGGCAAACAAGT CAGCGTGTCTACCGATTTCA 40.0 50.0 57.9 58.0 741-1044 bp 0.887097 86.7
rpl32-trnL-UAG_p3 TTTTTGTGCGGCAAACAAGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.9 57.9 750-1053 bp 0.887097 85.8
rpl32-trnL-UAG_p4 TTTTTGTGCGGCAAACAAGT GCTATCATCCCCTTTTCCGG 40.0 55.0 57.9 58.1 1014-1318 bp 0.822581 83.4
rpl32-trnL-UAG_p5 TTTTTGTGCGGCAAACAAGT CTATCATCCCCTTTTCCGGC 40.0 55.0 57.9 58.1 1013-1317 bp 0.790323 82.1