Hotspot

ndhF-rpl32

SSC · 109871-110769 · 899 bp

Back to Iris

Pi 0.0452
MarkerSeek score 73.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
899
variable_sites
251
pi
0.0452
unique_haplotype_count
48
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACACATTCCCACAAGTTCCC TTACTTGTTTGCCGCACAAA 50.0 40.0 58.0 57.1 1192-1672 bp 0.790323 76.0
ndhF-rpl32_p2 ACACATTCCCACAAGTTCCC TTTACTTGTTTGCCGCACAA 50.0 40.0 58.0 57.1 1193-1673 bp 0.790323 76.0
ndhF-rpl32_p3 ACACATTCCCACAAGTTCCC TTTTACTTGTTTGCCGCACA 50.0 40.0 58.0 57.1 1194-1674 bp 0.790323 76.0
ndhF-rpl32_p4 CACATTCCCACAAGTTCCCA TTACTTGTTTGCCGCACAAA 50.0 40.0 58.0 57.1 1191-1671 bp 0.790323 76.0
ndhF-rpl32_p5 CACATTCCCACAAGTTCCCA TTTACTTGTTTGCCGCACAA 50.0 40.0 58.0 57.1 1192-1672 bp 0.790323 76.0