Hotspot

trnT-UGU-trnL-UAA

LSC · 46480-47475 · 996 bp

Back to Iris

Pi 0.0363
MarkerSeek score 74.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
996
variable_sites
230
pi
0.0363
unique_haplotype_count
54
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CGAAGCCATACCCAATCGAA ACCAATTTCGCCATATCCCC 50.0 50.0 58.1 58.0 1484-1811 bp 1.0 91.5
trnT-UGU-trnL-UAA_p2 AATTGTCGCTCTGTCAGACC ACCAATTTCGCCATATCCCC 50.0 50.0 57.9 58.0 1372-1699 bp 1.0 91.3
trnT-UGU-trnL-UAA_p3 CGAAGCCATACCCAATCGAA CTCAGGATTGCCCCCTTTTT 50.0 50.0 58.1 58.1 1764-1913 bp 0.516129 71.8
trnT-UGU-trnL-UAA_p4 CGAAGCCATACCCAATCGAA AACCCGTGTTCGTTTTCTCA 50.0 45.0 58.1 58.0 1826-1913 bp 0.467742 66.2
trnT-UGU-trnL-UAA_p5 CGAAGCCATACCCAATCGAA ACCCGTGTTCGTTTTCTCAA 50.0 45.0 58.1 58.0 1825-1912 bp 0.467742 66.2