Hotspot

trnD-GUC-trnY-GUA

LSC · 30547-30929 · 383 bp

Back to Iris

Pi 0.0446
MarkerSeek score 73.20
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
383
variable_sites
105
pi
0.0446
unique_haplotype_count
39
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 55.0 55.0 57.9 58.4 503-543 bp 1.0 88.6
trnD-GUC-trnY-GUA_p2 GGATCCTAGTTCGGGACTGA GGGACGGACTGTAAATTCGT 55.0 50.0 57.9 57.6 502-542 bp 1.0 88.4
trnD-GUC-trnY-GUA_p3 GGATCCTAGTTCGGGACTGA CGGACTGTAAATTCGTTGGC 55.0 50.0 57.9 57.5 498-538 bp 0.967742 86.0
trnD-GUC-trnY-GUA_p4 GGATCCTAGTTCGGGACTGA GGACTGTAAATTCGTTGGCG 55.0 50.0 57.9 57.5 497-537 bp 0.967742 86.0
trnD-GUC-trnY-GUA_p5 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 50.0 55.0 58.5 58.4 448-488 bp 1.0 85.4