Hotspot

psbM-trnD-GUC

LSC · 29630-30472 · 843 bp

Back to Iris

Pi 0.0308
MarkerSeek score 75.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
843
variable_sites
171
pi
0.0308
unique_haplotype_count
49
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TTGGCTGACCGTTTTTACGT CATATACCTCGCCGGGATTG 45.0 55.0 58.3 58.0 885-1048 bp 0.870968 84.1
psbM-trnD-GUC_p2 TGGCTGACCGTTTTTACGTA CATATACCTCGCCGGGATTG 45.0 55.0 57.5 58.0 884-1047 bp 0.870968 82.6
psbM-trnD-GUC_p3 TTGGCTGACCGTTTTTACGT TATACCTCGCCGGGATTGTA 45.0 50.0 58.3 57.7 883-1046 bp 0.870968 81.8
psbM-trnD-GUC_p4 TTGGCTGACCGTTTTTACGT ATATACCTCGCCGGGATTGT 45.0 50.0 58.3 58.4 884-1047 bp 0.870968 81.3
psbM-trnD-GUC_p5 TTTGGCTGACCGTTTTTACG CATATACCTCGCCGGGATTG 45.0 55.0 57.2 58.0 886-1049 bp 0.870968 80.7