Hotspot

petN-psbM

LSC · 28628-29524 · 897 bp

Back to Iris

Pi 0.0305
MarkerSeek score 75.30
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
897
variable_sites
194
pi
0.0305
unique_haplotype_count
45
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACGGTCAGCCAA 50.0 45.0 58.2 58.3 885-1027 bp 1.0 88.1
petN-psbM_p2 AGTATGGGGAAGGAGTGGAC ACGTAAAAACGGTCAGCCAA 55.0 45.0 58.1 58.3 827-969 bp 0.935484 85.8
petN-psbM_p3 GTATGGGGAAGGAGTGGACT ACGTAAAAACGGTCAGCCAA 55.0 45.0 58.1 58.3 826-968 bp 0.935484 85.8
petN-psbM_p4 GGGGAAGGAGTGGACTCTAG ACGTAAAAACGGTCAGCCAA 60.0 45.0 58.2 58.3 822-964 bp 0.951613 85.7
petN-psbM_p5 TATGGGGAAGGAGTGGACTC ACGTAAAAACGGTCAGCCAA 55.0 45.0 57.8 58.3 825-967 bp 0.935484 85.3