Hotspot

rps16-trnQ-UUG

LSC · 5802-6678 · 877 bp

Back to Iris

Pi 0.0406
MarkerSeek score 73.00
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
877
variable_sites
219
pi
0.0406
unique_haplotype_count
50
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACCCTAGATTTTTCCCCCGA AACGGGGAAATAAGCGGATC 50.0 50.0 58.0 58.0 1658-1887 bp 0.322581 59.9
rps16-trnQ-UUG_p2 ACCCTAGATTTTTCCCCCGA GAACGGGGAAATAAGCGGAT 50.0 50.0 58.0 58.0 1659-1888 bp 0.322581 59.9
rps16-trnQ-UUG_p3 ACCCTAGATTTTTCCCCCGA TCAGAACGGGGAAATAAGCG 50.0 50.0 58.0 58.0 1662-1891 bp 0.306452 59.3
rps16-trnQ-UUG_p4 TGTTCCGGAATCCTGTCTCT TCAGAACGGGGAAATAAGCG 50.0 50.0 58.1 58.0 1952-2191 bp 0.306452 59.0
rps16-trnQ-UUG_p5 ACCCTAGATTTTTCCCCCGA CGGGGAAATAAGCGGATCAA 50.0 50.0 58.0 58.0 1656-1885 bp 0.274194 57.4