Hotspot

trnK-UUU-rps16

LSC · 4043-4693 · 651 bp

Back to Iris

Pi 0.0385
MarkerSeek score 72.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
651
variable_sites
179
pi
0.0385
unique_haplotype_count
49
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CTCTGCCGTTGAGTTAGCAA AGAGACAGGATTCCGGAACA 50.0 50.0 58.2 58.1 1055-1282 bp 0.790323 81.5
trnK-UUU-rps16_p2 ATGGTCCACTCACAGGAGAA TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.0 57.9 1105-1318 bp 0.677419 77.7
trnK-UUU-rps16_p3 ATGGTCCACTCACAGGAGAA TCGAGCCGTATGAGGAGAAA 50.0 50.0 58.0 58.2 1207-1420 bp 0.677419 76.9
trnK-UUU-rps16_p4 ATGGTCCACTCACAGGAGAA AGAGACAGGATTCCGGAACA 50.0 50.0 58.0 58.1 1506-1713 bp 0.532258 72.6
trnK-UUU-rps16_p5 ATGGTCCACTCACAGGAGAA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.0 58.2 1105-1212 bp 0.548387 72.2