primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score trnK-UUU-rps16_p1 trnK-UUU-rps16 1 CTCTGCCGTTGAGTTAGCAA AGAGACAGGATTCCGGAACA 20 20 50.0 50.0 58.2 58.1 0.0 21.6 0.265648 4043 4693 1055-1282 0.790323 81.5 trnK-UUU-rps16_p2 trnK-UUU-rps16 2 ATGGTCCACTCACAGGAGAA TCGATCCCGAAGAGAAGGAA 20 20 50.0 50.0 58.0 57.9 0.0 0.0 0.169351 4043 4693 1105-1318 0.677419 77.7 trnK-UUU-rps16_p3 trnK-UUU-rps16 3 ATGGTCCACTCACAGGAGAA TCGAGCCGTATGAGGAGAAA 20 20 50.0 50.0 58.0 58.2 0.0 0.0 0.271091 4043 4693 1207-1420 0.677419 76.9 trnK-UUU-rps16_p4 trnK-UUU-rps16 4 ATGGTCCACTCACAGGAGAA AGAGACAGGATTCCGGAACA 20 20 50.0 50.0 58.0 58.1 0.0 21.6 0.091443 4043 4693 1506-1713 0.532258 72.6 trnK-UUU-rps16_p5 trnK-UUU-rps16 5 ATGGTCCACTCACAGGAGAA GGTGCTCAACCTACAGGAAC 20 20 50.0 55.0 58.0 58.2 0.0 1.3 0.220343 4043 4693 1105-1212 0.548387 72.2 rps16-trnQ-UUG_p1 rps16-trnQ-UUG 1 ACCCTAGATTTTTCCCCCGA AACGGGGAAATAAGCGGATC 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.061357 5802 6678 1658-1887 0.322581 59.9 rps16-trnQ-UUG_p2 rps16-trnQ-UUG 2 ACCCTAGATTTTTCCCCCGA GAACGGGGAAATAAGCGGAT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.061357 5802 6678 1659-1888 0.322581 59.9 rps16-trnQ-UUG_p3 rps16-trnQ-UUG 3 ACCCTAGATTTTTCCCCCGA TCAGAACGGGGAAATAAGCG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.043071 5802 6678 1662-1891 0.306452 59.3 rps16-trnQ-UUG_p4 rps16-trnQ-UUG 4 TGTTCCGGAATCCTGTCTCT TCAGAACGGGGAAATAAGCG 20 20 50.0 50.0 58.1 58.0 22.0 0.0 0.079957 5802 6678 1952-2191 0.306452 59.0 rps16-trnQ-UUG_p5 rps16-trnQ-UUG 5 ACCCTAGATTTTTCCCCCGA CGGGGAAATAAGCGGATCAA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.062736 5802 6678 1656-1885 0.274194 57.4 psbK-psbI_p1 psbK-psbI 1 GTTTGGCAAGCTGCTGTAAG ACAAAGAGTTTGAGAGTAAGCA 20 22 50.0 36.4 57.9 55.6 1.4 6.4 4.446983 7267 7652 120-460 1.000000 56.4 psbK-psbI_p2 psbK-psbI 2 TGGCAAGCTGCTGTAAGTTT ACAAAGAGTTTGAGAGTAAGCA 20 22 45.0 36.4 58.3 55.6 1.4 6.4 4.699693 7267 7652 117-457 1.000000 54.4 psbK-psbI_p3 psbK-psbI 3 TTGGCAAGCTGCTGTAAGTT ACAAAGAGTTTGAGAGTAAGCA 20 22 45.0 36.4 58.3 55.6 1.4 6.4 4.699693 7267 7652 118-458 1.000000 54.4 psbK-psbI_p4 psbK-psbI 4 TTTGGCAAGCTGCTGTAAGT ACAAAGAGTTTGAGAGTAAGCA 20 22 45.0 36.4 58.3 55.6 1.4 6.4 4.699693 7267 7652 119-459 1.000000 54.4 psbK-psbI_p5 psbK-psbI 5 GTTTGGCAAGCTGCTGTAAG AACAAAGAGTTTGAGAGTAAGCA 20 23 50.0 34.8 57.9 56.3 1.4 6.4 4.722713 7267 7652 121-461 1.000000 54.2 trnS-GCU-trnG-UCC_p1 trnS-GCU-trnG-UCC 1 AATCTGCCGCTTTAGTCCAC ACGAATCACACTTTTACCACT 20 21 50.0 38.1 58.3 55.5 0.0 0.0 3.796776 7978 8866 914-957 1.000000 61.6 trnS-GCU-trnG-UCC_p2 trnS-GCU-trnG-UCC 2 CAATCTGCCGCTTTAGTCCA ACGAATCACACTTTTACCACT 20 21 50.0 38.1 58.3 55.5 0.0 0.0 3.798004 7978 8866 915-958 1.000000 61.6 trnS-GCU-trnG-UCC_p3 trnS-GCU-trnG-UCC 3 CGGAGAGAGAGGGATTCGAA ACGAATCACACTTTTACCACT 20 21 55.0 38.1 58.3 55.5 0.0 0.0 3.854304 7978 8866 968-1011 1.000000 61.2 trnS-GCU-trnG-UCC_p4 trnS-GCU-trnG-UCC 4 AATCTGCCGCTTTAGTCCAC AGAACGAATCACACTTTTACCAC 20 23 50.0 39.1 58.3 57.3 0.0 0.0 3.929491 7978 8866 917-960 1.000000 60.6 trnS-GCU-trnG-UCC_p5 trnS-GCU-trnG-UCC 5 AATCTGCCGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 20 23 50.0 39.1 58.3 57.3 0.0 0.0 3.929491 7978 8866 916-959 1.000000 60.6 trnG-UCC-trnR-UCU_p1 trnG-UCC-trnR-UCU 1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 20 21 50.0 47.6 58.0 56.9 0.0 0.0 2.081443 9611 9747 209-222 1.000000 75.3 trnG-UCC-trnR-UCU_p2 trnG-UCC-trnR-UCU 2 CCTAGCCTTCCAAGCTAACG AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 58.1 56.9 0.0 0.0 2.110280 9611 9747 212-225 1.000000 75.1 trnG-UCC-trnR-UCU_p3 trnG-UCC-trnR-UCU 3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 20 22 50.0 45.5 58.0 57.9 0.0 0.0 2.164771 9611 9747 215-228 1.000000 74.7 trnG-UCC-trnR-UCU_p4 trnG-UCC-trnR-UCU 4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTCTGTCCT 20 22 55.0 45.5 58.1 57.9 0.0 0.0 2.193608 9611 9747 218-231 1.000000 74.5 trnG-UCC-trnR-UCU_p5 trnG-UCC-trnR-UCU 5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTCTGTCCTATCCA 20 21 55.0 47.6 57.7 56.9 0.0 0.0 2.385293 9611 9747 213-226 1.000000 72.9 rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 CCAAATTAATCCCGCGGGTA CGGGGCAAGAATTCGTCTAA 20 20 50.0 50.0 58.0 58.0 41.6 0.0 0.048450 26175 27377 1237-1637 0.870968 86.5 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 TCGAGGGCTCTACCAATTGA CGGGGCAAGAATTCGTCTAA 20 20 50.0 50.0 58.1 58.0 7.1 0.0 0.157901 26175 27377 1215-1563 0.758065 81.1 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 CCAAATTAATCCCGCGGGTA CCGAACTCCGACTTTCGTAG 20 20 50.0 55.0 58.0 58.1 41.6 10.1 0.137441 26175 27377 1334-1902 0.741935 80.6 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 CCAAATTAATCCCGCGGGTA ACCAAGATTTCCGAACTCCG 20 20 50.0 50.0 58.0 57.9 41.6 0.0 0.124670 26175 27377 1344-1912 0.435484 68.4 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 CCAAATTAATCCCGCGGGTA GGATTTGAACCGGGGATGAA 20 20 50.0 50.0 58.0 57.9 41.6 6.3 0.167718 26175 27377 1524-1536 0.112903 45.5 petN-psbM_p1 petN-psbM 1 TAGTAAGTCTCGCTTGGGCT ACGTAAAAACGGTCAGCCAA 20 20 50.0 45.0 58.2 58.3 0.0 2.8 0.485294 28628 29524 885-1027 1.000000 88.1 petN-psbM_p2 petN-psbM 2 AGTATGGGGAAGGAGTGGAC ACGTAAAAACGGTCAGCCAA 20 20 55.0 45.0 58.1 58.3 0.0 2.8 0.453005 28628 29524 827-969 0.935484 85.8 petN-psbM_p3 petN-psbM 3 GTATGGGGAAGGAGTGGACT ACGTAAAAACGGTCAGCCAA 20 20 55.0 45.0 58.1 58.3 0.0 2.8 0.453005 28628 29524 826-968 0.935484 85.8 petN-psbM_p4 petN-psbM 4 GGGGAAGGAGTGGACTCTAG ACGTAAAAACGGTCAGCCAA 20 20 60.0 45.0 58.2 58.3 6.7 2.8 0.550875 28628 29524 822-964 0.951613 85.7 petN-psbM_p5 petN-psbM 5 TATGGGGAAGGAGTGGACTC ACGTAAAAACGGTCAGCCAA 20 20 55.0 45.0 57.8 58.3 0.0 2.8 0.510194 28628 29524 825-967 0.935484 85.3 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 TTGGCTGACCGTTTTTACGT CATATACCTCGCCGGGATTG 20 20 45.0 55.0 58.3 58.0 0.0 2.2 0.344091 29630 30472 885-1048 0.870968 84.1 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 TGGCTGACCGTTTTTACGTA CATATACCTCGCCGGGATTG 20 20 45.0 55.0 57.5 58.0 0.0 2.2 0.532981 29630 30472 884-1047 0.870968 82.6 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 TTGGCTGACCGTTTTTACGT TATACCTCGCCGGGATTGTA 20 20 45.0 50.0 58.3 57.7 0.0 2.2 0.625155 29630 30472 883-1046 0.870968 81.8 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 TTGGCTGACCGTTTTTACGT ATATACCTCGCCGGGATTGT 20 20 45.0 50.0 58.3 58.4 0.0 2.2 0.693030 29630 30472 884-1047 0.870968 81.3 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 TTTGGCTGACCGTTTTTACG CATATACCTCGCCGGGATTG 20 20 45.0 55.0 57.2 58.0 0.0 2.2 0.769683 29630 30472 886-1049 0.870968 80.7 trnD-GUC-trnY-GUA_p1 trnD-GUC-trnY-GUA 1 GGATCCTAGTTCGGGACTGA GGGGACGGACTGTAAATTCG 20 20 55.0 55.0 57.9 58.4 3.8 0.0 0.420330 30547 30929 503-543 1.000000 88.6 trnD-GUC-trnY-GUA_p2 trnD-GUC-trnY-GUA 2 GGATCCTAGTTCGGGACTGA GGGACGGACTGTAAATTCGT 20 20 55.0 50.0 57.9 57.6 3.8 0.0 0.444007 30547 30929 502-542 1.000000 88.4 trnD-GUC-trnY-GUA_p3 trnD-GUC-trnY-GUA 3 GGATCCTAGTTCGGGACTGA CGGACTGTAAATTCGTTGGC 20 20 55.0 50.0 57.9 57.5 3.8 0.0 0.589520 30547 30929 498-538 0.967742 86.0 trnD-GUC-trnY-GUA_p4 trnD-GUC-trnY-GUA 4 GGATCCTAGTTCGGGACTGA GGACTGTAAATTCGTTGGCG 20 20 55.0 50.0 57.9 57.5 3.8 0.0 0.589520 30547 30929 497-537 0.967742 86.0 trnD-GUC-trnY-GUA_p5 trnD-GUC-trnY-GUA 5 CGGTGCTCTGACCAATTGAA GGGGACGGACTGTAAATTCG 20 20 50.0 55.0 58.5 58.4 7.1 0.0 0.828828 30547 30929 448-488 1.000000 85.4 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 CGAAGCCATACCCAATCGAA ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 58.1 58.0 0.0 0.0 0.056519 46480 47475 1484-1811 1.000000 91.5 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 AATTGTCGCTCTGTCAGACC ACCAATTTCGCCATATCCCC 20 20 50.0 50.0 57.9 58.0 0.0 0.0 0.090678 46480 47475 1372-1699 1.000000 91.3 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 CGAAGCCATACCCAATCGAA CTCAGGATTGCCCCCTTTTT 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.109119 46480 47475 1764-1913 0.516129 71.8 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 CGAAGCCATACCCAATCGAA AACCCGTGTTCGTTTTCTCA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.078899 46480 47475 1826-1913 0.467742 66.2 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 CGAAGCCATACCCAATCGAA ACCCGTGTTCGTTTTCTCAA 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.078899 46480 47475 1825-1912 0.467742 66.2 trnL-UAA-trnF-GAA_p1 trnL-UAA-trnF-GAA 1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.5 58.1 0.0 20.1 0.526305 47959 48340 423-493 1.000000 87.8 trnL-UAA-trnF-GAA_p2 trnL-UAA-trnF-GAA 2 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 20 20 50.0 50.0 57.5 57.5 0.0 20.1 0.931776 47959 48340 420-490 1.000000 84.5 trnL-UAA-trnF-GAA_p3 trnL-UAA-trnF-GAA 3 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 20 20 50.0 50.0 57.0 58.1 0.0 20.1 1.013432 47959 48340 412-482 1.000000 83.9 trnL-UAA-trnF-GAA_p4 trnL-UAA-trnF-GAA 4 AATCGTGAGGGTTCAAGTCC TCCTCTGCTCTACCAACTGA 20 20 50.0 50.0 57.5 57.4 0.0 0.0 1.079322 47959 48340 395-465 1.000000 83.4 trnL-UAA-trnF-GAA_p5 trnL-UAA-trnF-GAA 5 AATCGTGAGGGTTCAAGTCC TACCAACTGAGCTATCCCGA 20 20 50.0 50.0 57.5 57.6 0.0 0.0 0.921818 47959 48340 385-455 0.919355 81.4 petA-psbJ_p1 petA-psbJ 1 TGAAAAGGTTCAATTGTACGA CATATTCCGGGTTGGGTTCA 21 20 33.3 50.0 53.7 57.6 5.1 0.0 5.739256 61748 62593 724-922 1.000000 52.0 petA-psbJ_p2 petA-psbJ 2 TGAAAAGGTTCAATTGTACGA GGTTCATATTCCGGGTTGGG 21 20 33.3 55.0 53.7 58.3 5.1 0.0 5.623149 61748 62593 728-915 0.967742 50.7 petA-psbJ_p3 petA-psbJ 3 AGAAACAGTTTGAAAAGGTTCA GGTTCATATTCCGGGTTGGG 22 20 31.8 55.0 54.6 58.3 2.3 0.0 5.705610 61748 62593 738-925 0.967742 50.7 petA-psbJ_p4 petA-psbJ 4 TGAAAAGGTTCAATTGTACGA GTTCATATTCCGGGTTGGGT 21 20 33.3 50.0 53.7 57.6 5.1 0.0 5.740960 61748 62593 727-914 0.967742 50.7 petA-psbJ_p5 petA-psbJ 5 TGAAAAGGTTCAATTGTACGAA GGTTCATATTCCGGGTTGGG 22 20 31.8 55.0 54.5 58.3 5.1 0.0 5.785088 61748 62593 728-915 0.967742 50.7 psbE-petL_p1 psbE-petL 1 CAACCCGCAATGAATAGGGA CAATCAACAGAGCTGGGGTT 20 20 50.0 50.0 57.9 58.1 6.4 0.0 0.136193 63363 64560 1147-1399 1.000000 90.9 psbE-petL_p2 psbE-petL 2 ACGAATAACCAACCCGCAAT CAATCAACAGAGCTGGGGTT 20 20 45.0 50.0 58.2 58.1 0.0 0.0 0.255880 63363 64560 1156-1408 1.000000 90.0 psbE-petL_p3 psbE-petL 3 CGAATAACCAACCCGCAATG CAATCAACAGAGCTGGGGTT 20 20 50.0 50.0 57.8 58.1 0.0 0.0 0.272210 63363 64560 1155-1407 1.000000 89.8 psbE-petL_p4 psbE-petL 4 ACCAACCCGCAATGAATAGG CAATCAACAGAGCTGGGGTT 20 20 50.0 50.0 58.2 58.1 0.0 0.0 0.322152 63363 64560 1149-1401 1.000000 89.4 psbE-petL_p5 psbE-petL 5 CCGCAATGAATAGGGAAGGT CAATCAACAGAGCTGGGGTT 20 20 50.0 50.0 57.7 58.1 0.0 0.0 0.433270 63363 64560 1143-1395 1.000000 88.5 ndhF-rpl32_p1 ndhF-rpl32 1 ACACATTCCCACAAGTTCCC TTACTTGTTTGCCGCACAAA 20 20 50.0 40.0 58.0 57.1 0.0 0.0 0.946077 109871 110769 1192-1672 0.790323 76.0 ndhF-rpl32_p2 ndhF-rpl32 2 ACACATTCCCACAAGTTCCC TTTACTTGTTTGCCGCACAA 20 20 50.0 40.0 58.0 57.1 0.0 0.0 0.946077 109871 110769 1193-1673 0.790323 76.0 ndhF-rpl32_p3 ndhF-rpl32 3 ACACATTCCCACAAGTTCCC TTTTACTTGTTTGCCGCACA 20 20 50.0 40.0 58.0 57.1 0.0 0.0 0.946077 109871 110769 1194-1674 0.790323 76.0 ndhF-rpl32_p4 ndhF-rpl32 4 CACATTCCCACAAGTTCCCA TTACTTGTTTGCCGCACAAA 20 20 50.0 40.0 58.0 57.1 0.0 0.0 0.947497 109871 110769 1191-1671 0.790323 76.0 ndhF-rpl32_p5 ndhF-rpl32 5 CACATTCCCACAAGTTCCCA TTTACTTGTTTGCCGCACAA 20 20 50.0 40.0 58.0 57.1 0.0 0.0 0.947497 109871 110769 1192-1672 0.790323 76.0 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 TTTTTGTGCGGCAAACAAGT AGCGTGTCTACCGATTTCAC 20 20 40.0 50.0 57.9 58.0 3.2 0.0 0.093885 110944 111688 740-1043 0.887097 86.7 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 TTTTTGTGCGGCAAACAAGT CAGCGTGTCTACCGATTTCA 20 20 40.0 50.0 57.9 58.0 3.2 0.0 0.095255 110944 111688 741-1044 0.887097 86.7 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 TTTTTGTGCGGCAAACAAGT TCCTAAGAGCAGCGTGTCTA 20 20 40.0 50.0 57.9 57.9 3.2 0.0 0.210332 110944 111688 750-1053 0.887097 85.8 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 TTTTTGTGCGGCAAACAAGT GCTATCATCCCCTTTTCCGG 20 20 40.0 55.0 57.9 58.1 3.2 0.0 0.188494 110944 111688 1014-1318 0.822581 83.4 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 TTTTTGTGCGGCAAACAAGT CTATCATCCCCTTTTCCGGC 20 20 40.0 55.0 57.9 58.1 3.2 0.0 0.188494 110944 111688 1013-1317 0.790323 82.1 rps15-ycf1_p1 rps15-ycf1 1 ATTCAACAGACCCGCAGTTT GACGTCGTAGAGAGTGGAGA 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.026104 120879 121374 717-880 0.903226 87.9 rps15-ycf1_p2 rps15-ycf1 2 AATTCAACAGACCCGCAGTT GACGTCGTAGAGAGTGGAGA 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.026104 120879 121374 718-881 0.887097 87.3 rps15-ycf1_p3 rps15-ycf1 3 ATTCAACAGACCCGCAGTTT AGACGTCGTAGAGAGTGGAG 20 20 45.0 55.0 58.0 58.0 0.0 10.5 0.031680 120879 121374 718-881 0.887097 87.2 rps15-ycf1_p4 rps15-ycf1 4 AATTCAACAGACCCGCAGTT AGACGTCGTAGAGAGTGGAG 20 20 45.0 55.0 58.0 58.0 0.0 10.5 0.031680 120879 121374 719-882 0.870968 86.6 rps15-ycf1_p5 rps15-ycf1 5 AAATTCAACAGACCCGCAGT GACGTCGTAGAGAGTGGAGA 20 20 45.0 55.0 58.0 58.0 0.0 0.0 0.026104 120879 121374 821-882 0.258065 58.6 ycf1_p1 ycf1 1 CATACCACTCGGGGAATTGG TGAACGGACCATATCGAGGA 20 20 55.0 50.0 58.0 57.9 0.0 0.0 0.094191 124571 124690 969-1086 0.629032 76.4 ycf1_p2 ycf1 2 CATACCACTCGGGGAATTGG TGGAAAAACCGGACATTCGT 20 20 55.0 45.0 58.0 58.0 0.0 9.5 0.062590 124571 124690 600-606 0.080645 44.3 ycf1_p3 ycf1 3 CATACCACTCGGGGAATTGG GGATCGATGGAAAAACCGGA 20 20 55.0 50.0 58.0 58.0 0.0 9.5 0.065362 124571 124690 613-613 0.016129 40.1 ycf1_p4 ycf1 4 CATACCACTCGGGGAATTGG AGGGATCGATGGAAAAACCG 20 20 55.0 50.0 58.0 58.0 0.0 0.5 0.071068 124571 124690 615-615 0.016129 40.1 ycf1_p5 ycf1 5 CATACCACTCGGGGAATTGG ACCGGACATTCGTTCAAGAG 20 20 55.0 50.0 58.0 57.9 0.0 9.5 0.112967 124571 124690 599-599 0.016129 39.7