Hotspot

ndhA

SSC · 128575-131123 · 2549 bp

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Pi 0.0136

MarkerSeek score 69.30

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2549
variable_sites: 150
pi: 0.0136
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCTCTTGTTTGAGGCAAGCC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.4	57.9	2568-2623 bp	1.0	82.7
ndhA_p2	TCTCTTGTTTGAGGCAAGCC	GGGGGAAGTTGATCGTTGAA	50.0	50.0	58.4	57.8	2566-2621 bp	1.0	82.2
ndhA_p3	TCTCTTGTTTGAGGCAAGCC	GTCCACCGGGTTTGATCAAT	50.0	50.0	58.4	58.2	2666-2721 bp	0.95	80.1
ndhA_p4	GCAAGCCGTCAATCTTGAAT	ATGGGGGAAGTTGATCGTTG	45.0	50.0	57.1	57.9	2555-2610 bp	1.0	78.4
ndhA_p5	TCTCTTGTTTGAGGCAAGCC	TCCACCGGGTTTGATCAATT	50.0	45.0	58.4	57.4	2665-2720 bp	0.95	76.7