Hotspot

ycf2-trnI-CAU

IRa · 160899-161126 · 228 bp

Back to Ipomoea

Pi 0.0321

MarkerSeek score 59.60

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 228
variable_sites: 43
pi: 0.0321
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TGAATTGAATTGGGTCCACGA	ATTGAACCTACGACTTCGCC	42.9	50.0	57.9	58.0	286-636 bp	1.0	82.8
ycf2-trnI-CAU_p2	TGAATTGAATTGGGTCCACGA	GAACCTACGACTTCGCCAAT	42.9	50.0	57.9	58.0	283-633 bp	1.0	82.8
ycf2-trnI-CAU_p3	TGAATTGGGTCCACGAATCT	ATTGAACCTACGACTTCGCC	45.0	50.0	56.8	58.0	281-631 bp	1.0	82.6
ycf2-trnI-CAU_p4	TGAATTGGGTCCACGAATCT	GAACCTACGACTTCGCCAAT	45.0	50.0	56.8	58.0	278-628 bp	1.0	82.6
ycf2-trnI-CAU_p5	TGAATTGAATTGGGTCCACGA	TTATGAGTTGGGCGCTTTCA	42.9	45.0	57.9	57.8	264-614 bp	1.0	81.3