Hotspot

trnI-CAU-ycf2

IRb · 88076-88303 · 228 bp

Back to Ipomoea

Pi 0.034

MarkerSeek score 59.30

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 228
variable_sites: 43
pi: 0.034
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GAACCTACGACTTCGCCAAT	GACCCAACAGCTTCTTCCAA	50.0	50.0	58.0	58.0	564-914 bp	1.0	89.2
trnI-CAU-ycf2_p2	ATTGAACCTACGACTTCGCC	AAGAAAACCCCCGCTTCAAT	50.0	45.0	58.0	58.0	924-1274 bp	1.0	87.9
trnI-CAU-ycf2_p3	ATTGAACCTACGACTTCGCC	AGAAAACCCCCGCTTCAATT	50.0	45.0	58.0	58.0	923-1273 bp	1.0	87.9
trnI-CAU-ycf2_p4	GAACCTACGACTTCGCCAAT	AAGAAAACCCCCGCTTCAAT	50.0	45.0	58.0	58.0	921-1271 bp	1.0	87.9
trnI-CAU-ycf2_p5	GAACCTACGACTTCGCCAAT	AGAAAACCCCCGCTTCAATT	50.0	45.0	58.0	58.0	920-1270 bp	1.0	87.9