Hotspot

psaA-ycf3

LSC · 43367-44140 · 774 bp

Back to Ipomoea

Pi 0.00897

MarkerSeek score 66.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 31
pi: 0.00897
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGATGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.0	58.0	934-1011 bp	1.0	85.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGATGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	952-1029 bp	1.0	85.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGATGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.0	57.9	886-963 bp	1.0	85.3
psaA-ycf3_p4	GCCCATTCCTCGAAAGATGT	AGACTTGGTTCGATCAAGCC	50.0	50.0	58.0	57.9	964-1041 bp	1.0	85.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGATGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.0	58.1	954-1031 bp	1.0	85.0