Hotspot

trnS-UGA-lhbA

LSC · 37084-37440 · 357 bp

Back to Ipomoea

Pi 0.0148

MarkerSeek score 52.40

Primer available yes

Label trnS-UGA-lhbA

Feature Metadata

label_name: trnS-UGA-lhbA
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 14
pi: 0.0148
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-lhbA_p1	CAACCACTCAGCCATCTCTC	GTTACTTGACCAGCCCTCAG	55.0	55.0	58.0	57.9	480-488 bp	1.0	82.3
trnS-UGA-lhbA_p2	GTTTTCAAGACCGGGGCTAT	GTTACTTGACCAGCCCTCAG	50.0	55.0	57.9	57.9	500-508 bp	1.0	81.4
trnS-UGA-lhbA_p3	GCTATCAACCACTCAGCCAT	GTTACTTGACCAGCCCTCAG	50.0	55.0	57.7	57.9	485-493 bp	1.0	79.8
trnS-UGA-lhbA_p4	TCAACCACTCAGCCATCTCT	GTTACTTGACCAGCCCTCAG	50.0	55.0	58.4	57.9	481-489 bp	1.0	79.7
trnS-UGA-lhbA_p5	TTTTCAAGACCGGGGCTATC	GTTACTTGACCAGCCCTCAG	50.0	55.0	57.6	57.9	499-507 bp	1.0	79.1