Hotspot

psbC-trnS-UGA

LSC · 36749-36994 · 246 bp

Back to Ipomoea

Pi 0.0279

MarkerSeek score 64.60

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 16
pi: 0.0279
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	363-375 bp	1.0	84.2
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	343-355 bp	1.0	83.7
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	TCTTTCGGAGAGATGGCTGA	55.0	50.0	58.2	58.1	370-382 bp	1.0	83.1
psbC-trnS-UGA_p4	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	443-455 bp	1.0	82.9
psbC-trnS-UGA_p5	GCAGCCGGATTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.3	58.0	402-414 bp	1.0	82.7