Hotspot

trnC-GCA-petN

LSC · 28756-29665 · 910 bp

Back to Ipomoea

Pi 0.0116

MarkerSeek score 62.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 54
pi: 0.0116
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	972-991 bp	1.0	84.9
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCACTTCTTCCCCACACTAC	50.0	55.0	58.1	57.5	1028-1047 bp	1.0	81.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	971-990 bp	1.0	79.0
trnC-GCA-petN_p4	CCAGTTCAAATCCGGGTGTC	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.8	58.2	967-986 bp	1.0	78.9
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	980-999 bp	1.0	78.8