Hotspot

trnG-UCC-trnR-UCU

LSC · 9773-10299 · 527 bp

Back to Ipomoea

Pi 0.0316

MarkerSeek score 76.00

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 527
variable_sites: 81
pi: 0.0316
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	ACCAAAGTTTCATGCTCGGT	ACCTCTACCCTATCCATTAGGC	45.0	50.0	58.0	58.2	672-727 bp	1.0	74.2
trnG-UCC-trnR-UCU_p2	ACCAAAGTTTCATGCTCGGT	CCTCTACCCTATCCATTAGGCA	45.0	50.0	58.0	58.2	671-726 bp	1.0	74.2
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	ACCTCTACCCTATCCATTAGGC	50.0	50.0	58.0	58.2	602-657 bp	1.0	74.1
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	CCTCTACCCTATCCATTAGGCA	50.0	50.0	58.0	58.2	601-656 bp	1.0	74.1
trnG-UCC-trnR-UCU_p5	CCTAGCCTTCCAAGCTAACG	ACCTCTACCCTATCCATTAGGC	55.0	50.0	58.1	58.2	605-660 bp	1.0	73.9