Hotspot

trnH-GUG-psbA

LSC · 123-578 · 456 bp

Back to Ipomoea

Pi 0.029

MarkerSeek score 56.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 47
pi: 0.029
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.49
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CAATCCACCGCCTTAATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	349-589 bp	0.75	77.4
trnH-GUG-psbA_p2	AATCCACCGCCTTAATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	348-588 bp	0.75	77.4
trnH-GUG-psbA_p3	ACAATCCACCGCCTTAATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	350-590 bp	0.75	77.4
trnH-GUG-psbA_p4	CAATCCACCGCCTTAATCCA	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	350-590 bp	0.75	76.9
trnH-GUG-psbA_p5	AATCCACCGCCTTAATCCAC	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	349-589 bp	0.75	76.9