Hotspot

ycf1

SSC · 126757-132426 · 5670 bp

Back to Iochroma

Pi 0.00218

MarkerSeek score 37.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 32
pi: 0.00218
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCTTCTGCTATCGCTATCC	ATTAAAGGCTCTATGCGGGC	55.0	50.0	58.0	58.1	288 bp	1.0	80.8
ycf1_p2	GGAATGCTATCCCAGGCTTC	ATTAAAGGCTCTATGCGGGC	55.0	50.0	58.1	58.1	302 bp	1.0	80.0
ycf1_p3	GCGCAAAAGGAATGCTATCC	ATTAAAGGCTCTATGCGGGC	50.0	50.0	57.9	58.1	310 bp	1.0	79.7
ycf1_p4	GCTATCGCTATCCGTGCTTT	ATTAAAGGCTCTATGCGGGC	50.0	50.0	58.2	58.1	281 bp	1.0	79.2
ycf1_p5	GGCTTCTGCTATCGCTATCC	GCGCAAAACAGCTATTTGGA	55.0	45.0	58.0	57.7	262 bp	1.0	79.0