Hotspot

ndhF

SSC · 113003-115225 · 2223 bp

Back to Iochroma

Pi 0.00209

MarkerSeek score 43.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 10
pi: 0.00209
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGTGGAAAGTGAGGAAGA	TGAATCCCCGTTCGATTCAC	50.0	50.0	57.9	58.0	2729-2987 bp	1.0	83.4
ndhF_p2	AGGGTGGAAAGTGAGGAAGA	AATCCCCGTTCGATTCACTG	50.0	50.0	57.9	58.0	2727-2985 bp	1.0	83.3
ndhF_p3	ACTGCTTCCGAAATGAAGGG	TGAATCCCCGTTCGATTCAC	50.0	50.0	58.2	58.0	2690-2948 bp	1.0	82.9
ndhF_p4	ACTGCTTCCGAAATGAAGGG	AATCCCCGTTCGATTCACTG	50.0	50.0	58.2	58.0	2688-2946 bp	1.0	82.9
ndhF_p5	TAGGCAGAATACCCTCACCC	TGAATCCCCGTTCGATTCAC	55.0	50.0	58.2	58.0	2791-2943 bp	0.875	77.7