Hotspot

ndhD

SSC · 118371-119873 · 1503 bp

Back to Iochroma

Pi 0.000713

MarkerSeek score 28.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 3
pi: 0.000713
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGGGTCGTATTGGAATTGG	CAAATTGCTTCTGCTCCACG	55.0	50.0	58.3	58.0	2209-2211 bp	1.0	77.7
ndhD_p2	GGGGTCGTATTGGAATTGGG	CAAATTGCTTCTGCTCCACG	55.0	50.0	58.3	58.0	2208-2210 bp	1.0	77.7
ndhD_p3	GGGGGTCGTATTGGAATTGG	AATTGCTTCTGCTCCACGAA	55.0	45.0	58.3	58.1	2207-2209 bp	1.0	77.1
ndhD_p4	GGGGTCGTATTGGAATTGGG	AAATTGCTTCTGCTCCACGA	55.0	45.0	58.3	58.1	2207-2209 bp	1.0	77.1
ndhD_p5	GGGGTCGTATTGGAATTGGG	AATTGCTTCTGCTCCACGAA	55.0	45.0	58.3	58.1	2206-2208 bp	1.0	77.1