Hotspot

ndhF-rpl32

SSC · 115226-116009 · 784 bp

Back to Iochroma

Pi 0.00456

MarkerSeek score 34.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 784
variable_sites: 8
pi: 0.00456
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGTCGATCAAGTATCCGA	GCTCCAAATTTTGCATCGCT	50.0	45.0	57.9	58.0	1649-1667 bp	1.0	81.2
ndhF-rpl32_p2	GTGGGTCGATCAAGTATCCG	GCTCCAAATTTTGCATCGCT	55.0	45.0	57.9	58.0	1650-1668 bp	1.0	80.6
ndhF-rpl32_p3	GAATAAGTCCCGCGCCTATT	GCTCCAAATTTTGCATCGCT	50.0	45.0	57.8	58.0	1458-1476 bp	1.0	80.6
ndhF-rpl32_p4	TGGGTCGATCAAGTATCCGA	GCACCTATTTTGCATCGCTC	50.0	50.0	57.9	57.9	1665-1683 bp	1.0	80.4
ndhF-rpl32_p5	ATTGGGCGGATTTAGCAACT	GCTCCAAATTTTGCATCGCT	45.0	45.0	57.9	58.0	2106-2124 bp	1.0	80.2