Hotspot

ycf1-ndhF

SSC · 112983-113002 · 20 bp

Back to Iochroma

Pi 0.0762

MarkerSeek score 57.00

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 20
variable_sites: 3
pi: 0.0762
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	AGGGTGGAAAGTGAGGAAGA	TCAACCAAGAGGGAGGGAAT	50.0	50.0	57.9	57.9	761-1019 bp	1.0	83.3
ycf1-ndhF_p2	ACTGCTTCCGAAATGAAGGG	TCAACCAAGAGGGAGGGAAT	50.0	50.0	58.2	57.9	722-980 bp	1.0	82.9
ycf1-ndhF_p3	TAGGCAGAATACCCTCACCC	TCAACCAAGAGGGAGGGAAT	55.0	50.0	58.2	57.9	823-1081 bp	1.0	82.6
ycf1-ndhF_p4	AGGGTGGAAAGTGAGGAAGA	CCAAGAGGGAGGGAATTTGG	50.0	55.0	57.9	57.9	757-1015 bp	1.0	82.6
ycf1-ndhF_p5	AGGGTGGAAAGTGAGGAAGA	GCGGACGCATCTCTTCTTAT	50.0	50.0	57.9	57.9	460-572 bp	0.875	74.8