Hotspot

psbE-petL

LSC · 67771-68927 · 1157 bp

Back to Iochroma

Pi 0.0033

MarkerSeek score 46.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1157
variable_sites: 10
pi: 0.0033
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGA	45.0	45.0	58.0	57.0	1314 bp	1.0	72.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCCGCTAATAGAAAACCGA	50.0	45.0	57.9	57.0	1303 bp	1.0	72.4
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AGCCGCTAATAGAAAACCGA	55.0	45.0	58.2	57.0	1221 bp	1.0	71.3
psbE-petL_p4	ACCAACCCGCAATGAATAGG	AGCCGCTAATAGAAAACCGA	50.0	45.0	58.2	57.0	1305 bp	1.0	70.9
psbE-petL_p5	TGACAAATAACCAACCCGCA	AGCCGCTAATAGAAAACCGAA	45.0	42.9	58.0	57.7	1314 bp	1.0	70.4