Hotspot

accD-psaI

LSC · 61446-62195 · 750 bp

Back to Iochroma

Pi 0.00276

MarkerSeek score 52.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 4
pi: 0.00276
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGTTTATTCGACC	AAGAAGCCATTGCAAATGCC	47.6	45.0	57.4	57.9	936-945 bp	0.875	62.3
accD-psaI_p2	AGTTATTTAAGCTCCACGCCT	AAGAAGCCATTGCAAATGCC	42.9	45.0	57.4	57.9	876-885 bp	0.875	62.3
accD-psaI_p3	CCAGAAGGGTTTATTCGACCT	AAGAAGCCATTGCAAATGCC	47.6	45.0	57.4	57.9	935-944 bp	0.875	62.2
accD-psaI_p4	TCGACCTAATTGTACCACGT	AAGAAGCCATTGCAAATGCC	45.0	45.0	56.3	57.9	921-930 bp	0.875	61.8
accD-psaI_p5	TCCAGAAGGGTTTATTCGACC	TGCAAATGCCGGAAAGACTA	47.6	45.0	57.4	57.8	926-935 bp	0.875	61.6