Hotspot

trnY-GUA-trnT-GGU

LSC · 32203-33204 · 1002 bp

Back to Iochroma

Pi 0.00442

MarkerSeek score 59.10

Primer available yes

Label trnY-GUA-trnT-GGU

Feature Metadata

label_name: trnY-GUA-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 15
pi: 0.00442
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnT-GGU_p1	GCGGATTGGCGAATTATTGG	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1156-1160 bp	1.0	82.6
trnY-GUA-trnT-GGU_p2	GGCGGATTGGCGAATTATTG	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	1157-1161 bp	1.0	82.6
trnY-GUA-trnT-GGU_p3	GCGGATTGGCGAATTATTGG	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	1135-1139 bp	1.0	82.2
trnY-GUA-trnT-GGU_p4	GGCGGATTGGCGAATTATTG	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	1136-1140 bp	1.0	82.2
trnY-GUA-trnT-GGU_p5	GCGGATTGGCGAATTATTGG	TGGCGTTACTCTACCACTGA	50.0	50.0	57.9	58.1	1132-1136 bp	1.0	82.0