Hotspot

rpl32-trnL-UAG

SSC · 111217-112104 · 888 bp

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Pi 0.0243
MarkerSeek score 60.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
888
variable_sites
39
pi
0.0243
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 992-1003 bp 1.0 81.8
rpl32-trnL-UAG_p2 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 993-1004 bp 1.0 81.7
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.6 57.9 1002-1013 bp 1.0 80.8
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.6 57.8 1001-1012 bp 1.0 80.2
rpl32-trnL-UAG_p5 CGGCGTTAAAAGCTTTGTCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.6 57.8 1000-1011 bp 1.0 80.2