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rps8-rpl14

LSC · 79021-79235 · 215 bp

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Pi 0.00825
MarkerSeek score 61.30
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
215
variable_sites
3
pi
0.00825
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 ACTCGAACCACGCTTTTTCT TGCGATTGCCAGAGAATTGA 45.0 45.0 58.1 57.9 340-362 bp 1.0 83.4
rps8-rpl14_p2 ACTCGAACCACGCTTTTTCT GTGCGATTGCCAGAGAATTG 45.0 50.0 58.1 57.8 341-363 bp 1.0 82.8
rps8-rpl14_p3 ACTCGAACCACGCTTTTTCT AGAGTTTTTGGTGCGATTGC 45.0 45.0 58.1 57.6 351-373 bp 1.0 80.9
rps8-rpl14_p4 ACTCGAACCACGCTTTTTCT GAGTTTTTGGTGCGATTGCC 45.0 50.0 58.1 58.6 350-372 bp 1.0 79.6
rps8-rpl14_p5 ACTCGAACCACGCTTTTTCT GTTTCATTAGCTCCCGAGGT 45.0 50.0 58.1 57.3 297-319 bp 1.0 78.8