Hotspot

trnT-UGU-trnL-UAA

LSC · 45779-46343 · 565 bp

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Pi 0.0289
MarkerSeek score 65.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
565
variable_sites
32
pi
0.0289
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AAAACCTGACCTGTTGACCC CGGAAAACGTGATTTGGCTC 50.0 50.0 57.9 58.0 1306-1375 bp 1.0 86.0
trnT-UGU-trnL-UAA_p2 AATAGTCGGAGCCATACCCA CGGAAAACGTGATTTGGCTC 50.0 50.0 57.9 58.0 1370-1439 bp 1.0 85.6
trnT-UGU-trnL-UAA_p3 ATAGTCGGAGCCATACCCAA CGGAAAACGTGATTTGGCTC 50.0 50.0 57.9 58.0 1369-1438 bp 1.0 85.6
trnT-UGU-trnL-UAA_p4 AAAACCTGACCTGTTGACCC CTTCCATCGAGTCTCTGCAC 50.0 55.0 57.9 58.1 1368-1437 bp 1.0 85.5
trnT-UGU-trnL-UAA_p5 TGTTATCCAGGCGCATTTCA CGGAAAACGTGATTTGGCTC 45.0 50.0 57.9 58.0 1338-1407 bp 1.0 85.5