Hotspot

rps16-trnQ-UUG

LSC · 6329-7239 · 911 bp

Back to Inula

Pi 0.0216
MarkerSeek score 63.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
911
variable_sites
37
pi
0.0216
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CTAGATCCTTGCCCCTGAGA GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 1163-1176 bp 1.0 83.3
rps16-trnQ-UUG_p2 CTAGATCCTTGCCCCTGAGA CTTACAGCAGCTTGCCAAAC 55.0 50.0 57.9 57.9 1738-1751 bp 1.0 82.5
rps16-trnQ-UUG_p3 TTTATTCGGATCCGCGAGAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1481-1494 bp 1.0 82.2
rps16-trnQ-UUG_p4 ACTTTCTACTCGAGCTCCGT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.2 57.9 1707-1720 bp 1.0 81.9
rps16-trnQ-UUG_p5 TTTATTCGGATCCGCGAGAC CTTACAGCAGCTTGCCAAAC 50.0 50.0 57.9 57.9 2056-2069 bp 1.0 81.7