Hotspot

trnL-UAG

SSC · 134608-134687 · 80 bp

Back to Inga

Pi 0.526
MarkerSeek score 75.10
Primer available yes
Label trnL-UAG

Feature Metadata

label_name
trnL-UAG
feature_type
tRNA
region
SSC
length_bp
80
variable_sites
41
pi
0.526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG_p1 ACGGGGAACTCAAAAAGCTT AATGCGATTTTAGAGCGGGT 45.0 45.0 57.9 58.0 1094 bp 0.5 59.2
trnL-UAG_p2 ACGGGGAACTCAAAAAGCTT TGCGATTTTAGAGCGGGTAC 45.0 50.0 57.9 58.1 1092 bp 0.5 58.9
trnL-UAG_p3 ACGGGGAACTCAAAAAGCTT GCGATTTTAGAGCGGGTACT 45.0 50.0 57.9 57.8 1091 bp 0.5 57.7
trnL-UAG_p4 ACGGGGAACTCAAAAAGCTT TCGCAAAGCCTTGGGTAAAA 45.0 45.0 57.9 58.3 1031 bp 0.5 57.0
trnL-UAG_p5 ACGGGGAACTCAAAAAGCTT CAATGCGATTTTAGAGCGGG 45.0 50.0 57.9 57.6 1095 bp 0.5 56.3