Hotspot

ndhF

SSC · 130794-133031 · 2238 bp

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Pi 0.449
MarkerSeek score 77.60
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2238
variable_sites
778
pi
0.449
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.77
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AAAGGGTTCTCGACGGTTTG CCCGCTTACTTGGTCGTTAA 50.0 50.0 58.4 57.9 2966 bp 0.5 56.1
ndhF_p2 CTCGACGGTTTGGTTTATGC CCCGCTTACTTGGTCGTTAA 50.0 50.0 57.5 57.9 2958 bp 0.5 55.2
ndhF_p3 GGTTCTCGACGGTTTGGTTT CCCGCTTACTTGGTCGTTAA 50.0 50.0 58.7 57.9 2962 bp 0.5 53.8
ndhF_p4 AAAGGGTTCTCGACGGTTTG TATCCCGCTTACTTGGTCGT 50.0 50.0 58.4 58.5 2969 bp 0.5 52.5
ndhF_p5 AAAGGGTTCTCGACGGTTTG TTATCCCGCTTACTTGGTCG 50.0 50.0 58.4 57.4 2970 bp 0.5 52.0